Forskolin inhibition of cyclic AMP generation in J774 macrophages.

  title={Forskolin inhibition of cyclic AMP generation in J774 macrophages.},
  author={Anne-Marie Chambaut-Gu{\'e}rin and Pierre Thomopoulos},
  journal={European journal of pharmacology},
  volume={172 4-5},
Forskolin inhibited cyclic AMP generation in J774 macrophage cells in response to isoproterenol. Forskolin, 10 nM-0.1 mM, also inhibited the adenylate cyclase activity of membrane preparations. The basal activity and the isoproterenol-, cholera toxin-, fluoride- or GppNHp-stimulated activities were maximally depressed by 10 microM forskolin (30-70% inhibition, EC50 = 0.3-0.5 microM). This effect was achieved similarly in membranes from pertussis toxin-treated cells. Forskolin required guanine… CONTINUE READING