Formulation of highly soluble poly(ethylene glycol)-peptide DNA condensates.

@article{Kwok1999FormulationOH,
  title={Formulation of highly soluble poly(ethylene glycol)-peptide DNA condensates.},
  author={Kai Y Kwok and Donald L McKenzie and David L. Evers and Kevin G Rice},
  journal={Journal of pharmaceutical sciences},
  year={1999},
  volume={88 10},
  pages={996-1003}
}
Two poly(ethylene glycol) (PEG)-peptides were synthesized and tested for their ability to bind to plasmid DNA and form soluble DNA condensates with reduced spontaneous gene expression. PEG-vinyl sulfone or PEG-orthopyridyl disulfide were reacted with the sulfhydryl of Cys-Trp-Lys(18) (CWK(18)) resulting in the formation of nonreducible (PEG-VS-CWK(18)) and reducible (PEG-SS-CWK(18)) PEG-peptides. Both PEG-peptides were prepared on a micromole scale, purified by RP-HPLC in >80% yield, and… CONTINUE READING

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