Forms of LonB protease from Archaeoglobus fulgidus devoid of the transmembrane domain: The contribution of the quaternary structure to the regulation of enzyme proteolytic activity

@article{Makhovskaya2007FormsOL,
  title={Forms of LonB protease from Archaeoglobus fulgidus devoid of the transmembrane domain: The contribution of the quaternary structure to the regulation of enzyme proteolytic activity},
  author={Oksana V Makhovskaya and Serguei Kozlov and Istvan Botos and Anton A Stepnov and Anna G Andrianova and A. E. Gushchina and Alexander Wlodawer and E. E. Mel’nikov and Tatyana V. Rotanova},
  journal={Russian Journal of Bioorganic Chemistry},
  year={2007},
  volume={33},
  pages={610-613}
}
Deletion of the transmembrane domain (TM-domain) of Archaeoglobus fulgidus LonB protease (Archaeoglobus fulgidus (AfLon)) was shown to result in uncontrollable activation of the enzyme proteolytic site and in vivo autolysis yielding a stable and functionally inactive fragment consisting of both α-helical and proteolytic domains (αP). The ΔTM-AfLon-S509A… CONTINUE READING