Formation of the Apaf-1/cytochrome c complex precedes activation of caspase-9 during seizure-induced neuronal death

@article{Henshall2001FormationOT,
  title={Formation of the Apaf-1/cytochrome c complex precedes activation of caspase-9 during seizure-induced neuronal death},
  author={David C. Henshall and David P Bonislawski and Shana L. Skradski and Tomohiro Araki and J Q Lan and Clara Kay Schindler and Robert Meller and Roger Simon},
  journal={Cell Death and Differentiation},
  year={2001},
  volume={8},
  pages={1169-1181}
}
In this study we examine the in vivo formation of the Apaf-1/cytochrome c complex and activation of caspase-9 following limbic seizures in the rat. Seizures were elicited by unilateral intraamygdala microinjection of kainic acid to induce death of CA3 neurons within the hippocampus of the rat. Apaf-1 was found to interact with cytochrome c within the injured hippocampus 0–24 h following seizures by co-immunoprecipitation analysis and immunohistochemistry demonstrated Apaf-1/cytochrome c co… CONTINUE READING