Formation of supercoiling domains in plasmid pBR322.

  title={Formation of supercoiling domains in plasmid pBR322.},
  author={Jennifer K. Lodge and Toni Kazic and Douglas E. Berg},
  journal={Journal of bacteriology},
  volume={171 4},
Twin domains of positive and negative supercoiling are thought to form in DNA molecules whenever free rotation of a transcription complex around the DNA helix is impeded. Evidence for these domains has come from findings with Escherichia coli strains that are deficient in DNA topoisomerase I (top mutants) or that have been treated with DNA gyrase inhibitors. Plasmid pBR322 is highly supercoiled in these strains, whereas some of its deletion derivatives are not. The studies of pBR322 derivatives… CONTINUE READING

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