Formation of steady-state oxygen gradients in vitro: application to liver zonation.

@article{Allen2003FormationOS,
  title={Formation of steady-state oxygen gradients in vitro: application to liver zonation.},
  author={Jared W. Allen and Sangeeta N. Bhatia},
  journal={Biotechnology and bioengineering},
  year={2003},
  volume={82 3},
  pages={253-62}
}
We have developed a perfusion bioreactor system that allows the formation of steady state oxygen gradients in cell culture. In this study, gradients were formed in cultures of rat hepatocytes to study the role of oxygen in modulating cellular functions. A model of oxygen transport in our flat-plate reactor was developed to estimate oxygen distribution at the cell surface. Experimental measurements of outlet oxygen concentration from various flow conditions were used to validate model… CONTINUE READING