Formation of stable crystalline enzyme-substrate intermediates at sub-zero temperatures.

Abstract

Sub-zero temperatures can be used to trap intermediates in enzyme-catalysed reactions using suitable cryosolvents. The feasibility of obtaining such intermediates in the crystalline state for X-ray diffraction studies has been demonstrated with several proteases, using specific substrates and optimal pH.

Cite this paper

@article{Fink1976FormationOS, title={Formation of stable crystalline enzyme-substrate intermediates at sub-zero temperatures.}, author={Anthony L. Fink and A I Ahmed}, journal={Nature}, year={1976}, volume={263 5575}, pages={294-7} }