Formation of spicules by sclerocytes from the freshwater spongeEphydatia muelleri in short-term cultures in vitro

@article{Imsiecke1995FormationOS,
  title={Formation of spicules by sclerocytes from the freshwater spongeEphydatia muelleri in short-term cultures in vitro},
  author={Georg Imsiecke and Renate Steffen and M{\'a}rcio Reis Cust{\'o}dio and Radovan Borojevic and Werner E. G. M{\"u}ller},
  journal={In Vitro Cellular \& Developmental Biology - Animal},
  year={1995},
  volume={31},
  pages={528-535}
}
SummaryCells from the freshwater spongeEphydatia muelleri were isolated by dissociating hatching gemmules. During the first 24 h the cells reaggregated, but the aggregates progressively disintegrated again to single cells, among which the spicule-forming sclerocytes were recognized. Such cultures were used to study spicule (megascleres) formation in vitro. The isolated sclerocytes formed the organic central axial filament onto which they deposited inorganic silicon. The size of the spicules… 

Dynamics of spicule production in the marine sponge Hymeniacidon perlevis during in vitro cell culture and seasonal development in the field

Silicatein expression in the bloom stage was more than 100 times higher than that in the other stages and was correlated with the spicule developmental stage, and the trend of spicules formation in field-grown sponges was consistent with the trend in cell culture.

Analysis of silicatein gene expression and spicule formation in the demosponge Amphimedon queenslandica

Investigation of the process of spiculogenesis in the different developmental stages of the demosponge Amphimedon queenslandica, and the evolution and developmental expression of the silicatein gene family in relation to spicule formation, suggests that these genes have preserved an ancestral gene structure common to both families in both marine and freshwater sponges.

Evagination of Cells Controls Bio-Silica Formation and Maturation during Spicule Formation in Sponges

The enzymatic-silicatein mediated formation of the skeletal elements, the spicules of siliceous sponges starts intracellularly and is completed extracellularly, and the sequence of events that govern spicule formation is termed bio-inorganic self-organization.

Tropical freshwater sponges develop from gemmules faster than their temperate-region counterparts

The development during gemmule germination of two neotropical freshwater sponges, Radiospongilla inesi and Heteromeyenia cristalina is described and differences in the developmental stages between the studied species were observed, suggesting that development is species specific.

The biology of glass sponges.

The unique invention of the siliceous sponges: their enzymatically made bio-silica skeleton.

It is proposed that the key innovation that allowed the earliest metazoans to form larger specimens was the enzyme silicatein, crucial for the formation of the siliceous skeleton.

Purification and in vitro cultivation of archaeocytes (stem cells) of the marine sponge Hymeniacidon perleve (Demospongiae)

A novel four-step protocol for the purification of archaeocytes from a marine sponge, Hymeniacidon perleve, opens an important avenue towards developing sponge cell cultures for the commercial exploitation of sponge-derived drugs.

Sponge spicules as blueprints for the biofabrication of inorganic–organic composites and biomaterials

First bioinspired approaches implement recombinant silicatein and silintaphin-1 for applications in the field of biomedicine (biosilica-mediated regeneration of tooth and bone defects) or micro-optics (in vitro synthesis of light waveguides) with promising results.

Complex structures – smart solutions: Formation of siliceous spicules

It has now been found that the initial axial orientation, in which the spicules grow, is guided by cell processes through evagination, and highlights that for the ultimate determination of theSpicule shapes, their species-specific morphologies, bio-silica hardens during a process which removes reaction water.

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