Formation of spicules by sclerocytes from the freshwater spongeEphydatia muelleri in short-term cultures in vitro

@article{Imsiecke2007FormationOS,
  title={Formation of spicules by sclerocytes from the freshwater spongeEphydatia muelleri in short-term cultures in vitro},
  author={Georg Imsiecke and Renate Steffen and M{\'a}rcio Reis Cust{\'o}dio and Radovan Borojevic and Werner E. G. M{\"u}ller},
  journal={In Vitro Cellular \& Developmental Biology - Animal},
  year={2007},
  volume={31},
  pages={528-535}
}
SummaryCells from the freshwater spongeEphydatia muelleri were isolated by dissociating hatching gemmules. During the first 24 h the cells reaggregated, but the aggregates progressively disintegrated again to single cells, among which the spicule-forming sclerocytes were recognized. Such cultures were used to study spicule (megascleres) formation in vitro. The isolated sclerocytes formed the organic central axial filament onto which they deposited inorganic silicon. The size of the spicules… 

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