Formation of reduced nicotinamide adenine dinucleotide peroxide.

@article{Bernofsky1982FormationOR,
  title={Formation of reduced nicotinamide adenine dinucleotide peroxide.},
  author={C Bernofsky and S Y Wanda},
  journal={The Journal of biological chemistry},
  year={1982},
  volume={257 12},
  pages={6809-17}
}
Incubation of NADH at neutral and slightly alkaline pH leads to the gradual absorption of 1 mol of H+. This uptake of acid requires oxygen and mainly yields anomerized NAD+ (NAD+), with only minimal formation od acid-modified NADH. The overall stoichiometry of the reaction is: NADH + H+ + 1/2O2 leads to H2O + NAD+, with NADH peroxide (HO2-NADH+) serving as the intermediate that anomerizes and breaks down to give NAD+ and H2O2. The final reaction reaction mixture contains less than 0.1% of the… CONTINUE READING