Formation of protoplasts in Azotobacter vinelandii

Abstract

Protoplasts of Azotobacter vinelandii were formed by incubating whole cells in lysozyme and EDTA in Tris-HCl buffer (0.05 M, pH 8.0) supplemented with sucrose (15% w/v). This appeared to be related to the special chelating ability of EDTA and Tris-HCl since substitution of the former by nitrilotriacetic acid or by trisodium citrate and the latter by veronal… (More)
DOI: 10.1007/BF00455949

2 Figures and Tables

Topics

  • Presentations referencing similar topics