Formation of open and elongating transcription complexes by RNA polymerase III.

@article{Kassavetis1992FormationOO,
  title={Formation of open and elongating transcription complexes by RNA polymerase III.},
  author={George A. Kassavetis and Justin A. Blanco and Thomas Johnson and E. Peter Geiduschek},
  journal={Journal of molecular biology},
  year={1992},
  volume={226 1},
  pages={47-58}
}
The Saccharomyces cerevisiae transcription factors (TF) IIIB and IIIC assemble onto their respective DNA-binding sites on the SUP4 tRNA(Tyr) gene at 0 degrees C. RNA polymerase III specifically associates at 0 degrees C with this TFIIIC-TFIIIB-DNA complex to form a stable "closed" promoter complex in which the DNA surrounding the transcriptional start retains its duplex form. Promoter "opening" is a temperature-dependent and readily reversible process that involves up to 22 unwound base-pairs… CONTINUE READING