Formation of neuromuscular junctions in embryonic cell cultures.

  title={Formation of neuromuscular junctions in embryonic cell cultures.},
  author={Yutaka Shimada and Donald A. Fischman and A. A. Moscona},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={62 3},
The formation of nerve-muscle junctions in monolayer cultures of embryonic muscle and spinal cord cells is described. Muscle-forming cells (myoblasts) from leg muscles of 12-day chick embryos were separated with trypsin and cultured on a collagen substrate for two days. Suspensions of ventral spinal cord cells from six-day chick embryos were then plated over the differentiating muscle cells. The cultures were subsequently examined by electron microscopy and in silver-stained light microscopic… CONTINUE READING