Formation of linear inverted repeat amplicons following targeting of an essential gene in Leishmania

@article{Genest2005FormationOL,
  title={Formation of linear inverted repeat amplicons following targeting of an essential gene in Leishmania},
  author={Philippe Genest and Bas ter Riet and Carole Dumas and Barbara Papadopoulou and Henri G.A.M. van Luenen and Piet Borst},
  journal={Nucleic Acids Research},
  year={2005},
  volume={33},
  pages={1699 - 1709}
}
Attempts to inactivate an essential gene in the protozoan parasite Leishmania have often led to the generation of extra copies of the wild-type alleles of the gene. In experiments with Leishmania tarentolae set up to disrupt the gene encoding the J-binding protein 1 (JBP1), a protein binding to the unusual base beta-D-glucosyl-hydroxymethyluracil (J) of Leishmania, we obtained JBP1 mutants containing linear DNA elements (amplicons) of approximately 100 kb. These amplicons consist of a long… CONTINUE READING

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