Formation of ligand and metabolite complexes as a means for selective quantitation of cytochrome P450 isozymes.

@article{Roos1993FormationOL,
  title={Formation of ligand and metabolite complexes as a means for selective quantitation of cytochrome P450 isozymes.},
  author={Peter H. Roos and B Golub-Ciosk and P Kallweit and D Kauczinski and W G Hanstein},
  journal={Biochemical pharmacology},
  year={1993},
  volume={45 11},
  pages={2239-50}
}
The suitability of triacetyloleandomycin (TAO) metabolite complex formation and metyrapone binding to reduced cytochrome P450 as a means for selective isozyme quantitation has been studied. Although isozymes of both subfamilies bind metyrapone in the reduced state, selective quantitation of 2B isozymes through the metyrapone complex is possible after complex formation of P450 3A with a TAO metabolite. Thus, consecutive application of both reactions allows the spectroscopic quantitation of P450… CONTINUE READING
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