Formation of farnesal and 3-hydroxy-2,3-dihydrofarnesal from farnesol by protoplasts of Botryococcus braunii.

@article{Inoue1993FormationOF,
  title={Formation of farnesal and 3-hydroxy-2,3-dihydrofarnesal from farnesol by protoplasts of Botryococcus braunii.},
  author={Hitoshi Inoue and Tatsumi Korenaga and Hiroshi Sagami and Takako Koyama and Hiroshi Sugiyama and Kyozo Ogura},
  journal={Biochemical and biophysical research communications},
  year={1993},
  volume={196 3},
  pages={
          1401-5
        }
}
Farnesal and 3-hydroxy-2,3-dihydrofarnesal (3-hydroxy-3,7,11-trimethyl-6,10-dodecadiene-1-al) were formed from farnesol when the alcohol was incubated with the protoplast of Botryococcus braunii B race strain. This fact suggests the existence of farnesal hydratase in the alga. Feeding experiments showed that both farnesal and 3-hydroxy-2,3-dihydrofarnesal were efficiently incorporated into botryococcenes, triterpenoid hydrocarbons of the alga. 
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13 Citations

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Properties of farnesol phosphokinase of Botryococcus braunii
Biosynthesis of the triterpenoids, botryococcenes and tetramethylsqualene in the B race of Botryococcus braunii via the non-mevalonate pathway
Botryococcus braunii: a rich source for hydrocarbons and related ether lipids
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The algal biodiversity, the chemical structures and biosynthesis of hydrocarbons and ether lipids and the biotechnological studies related to hydrocarbon production are reviewed.
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This review covers recently-published experimental information on the biosynthesis of terpenoids in the range C5–C25 and considers in turn hemiterpenoids, polyprenyl diphosphate synthases, monoterpenoid, sesquiter penoids, diterPenoids, and sesterterpenoids.
Squalene – biochemistry, molecular biology, process biotechnology, and applications
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The recent knowledge of squalene biochemistry, its molecular properties, and its physiological effects are summarized and applications in nutrition, pharmacy, and medicine are discussed.
The history and recent advances in research of polyprenol and its derivatives
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This review focuses on the history and recent advances in research of polyprenol and its derivatives, including rubber-like prenol, epoxypoly prenol,Epoxydolichol, andpolyprenylacetone.
Molecular characterization of squalene synthase from the green microalga Botryococcus braunii, race B.
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Results suggest either that there exists coordinate expression of separate synthase genes for squalene and botryococcene biosynthesis or that there might be unique physiological conditions controlling the SS vs botryOCoccene synthase activity of a single peptide species.
Botryococcus braunii: A Renewable Source of Hydrocarbons and Other Chemicals
TLDR
This review details the various facets of biotechnology of B. braunii, including its microbiology and physiology; production of hydrocarbons and other compounds by the alga; methods of culture; downstream recovery and processing of algal hydrocarols; and cloning of the algal genes into other microorganisms.
Characterization of botryococcene synthase enzyme activity, a squalene synthase-like activity from the green microalga Botryococcus braunii, Race B.
Microalgae protoplasts isolation and fusion for biotechnology research
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The fusion of protoplasts in microalgae constitutes a tool for strain improvement because it allows both intra and interspecific genetic recombina-tion, resulting in organisms with new or improved characteristics of industrial interest.
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