Formation of enterovirus-like particle aggregates by recombinant baculoviruses co-expressing P1 and 3CD in insect cells

Abstract

The assembly of enterovirus requires the cleavage of P1 polyprotein by protease 3CD into individual structural proteins. Two recombinant baculoviruses were constructed to encode P1 and 3CD of enterovirus 71 (EV71), respectively. The expressed 3CD successfully cleaved P1 in vitro and in vivo. Also, the co-infection in insect cells resulted in crystalline virus-like particle structures morphologically resembling the authentic EV71 aggregates, which are reported for the first time.

DOI: 10.1023/A:1024071514438

3 Figures and Tables

050100150'05'06'07'08'09'10'11'12'13'14'15'16'17
Citations per Year

371 Citations

Semantic Scholar estimates that this publication has 371 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Hu2003FormationOE, title={Formation of enterovirus-like particle aggregates by recombinant baculoviruses co-expressing P1 and 3CD in insect cells}, author={Yu-Chen Hu and John Tsu-An Hsu and Jen-Huang Huang and Mei-Shang Ho and Yi-Chen Ho}, journal={Biotechnology Letters}, year={2003}, volume={25}, pages={919-925} }