Formation of coding joints in V(D)J recombination-inducible severe combined immune deficient pre-B cell lines.

@article{Chang1999FormationOC,
  title={Formation of coding joints in V(D)J recombination-inducible severe combined immune deficient pre-B cell lines.},
  author={Yung Chang and Matthew L. Brown},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1999},
  volume={96 1},
  pages={191-6}
}
Characterization of the severe combined immune deficient (scid) defect in the recombination process has provided many insights into the underlying mechanisms of variable (diversity) joining recombination. By using recombination-inducible scid pre-B cell lines transformed with the temperature-sensitive Abelson-murine leukemia virus, we show that large quantities of recombination intermediates can be generated, and their resolution can be followed during further cell culture. In this study, we… CONTINUE READING