Formation of callus and somatic embryos from protoplasts of a commercial hybrid of maize (Zea mays L.)

Abstract

Protoplasts isolated from a totipotent embryogenic cell suspension culture of Zea mays L. (cultivar ‘Dekalb XL82’) underwent sustained cell divisions when cultured in liquid as well as agarose media. Optimal colony formation (5%) occurred in a liquid medium containing 0.5 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D). A soft and unorganized callus was formed… (More)
DOI: 10.1007/BF00289381

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