Formation of bone and cartilage by marrow stromal cells in diffusion chambers in vivo.

@article{Ashton1980FormationOB,
  title={Formation of bone and cartilage by marrow stromal cells in diffusion chambers in vivo.},
  author={Brian A Ashton and Terrence D. Allen and Cameron Rolfe Howlett and C. C. Eaglesom and Akiko Hattori and Maureen Owen},
  journal={Clinical orthopaedics and related research},
  year={1980},
  volume={151},
  pages={294-307}
}
When freshly isolated rabbit marrow cells were cultured either in vitro or in diffusion chambers in vivo, the hemopoietic cells disappeared and there was a proliferation of the stromal cell population. The colonies formed in vitro were mainly fibroblastic, and this cell type predominated in confluent cultures. Staining for alkaline phosphatase activity and for the Von Kossa reaction was negative in in vitro cultures. However, marrow cell suspensions or fibroblasts harvested from in vitro… CONTINUE READING