Formation of azole-resistant Candida albicans by mutation of sterol 14-demethylase P450.

@article{Asai1999FormationOA,
  title={Formation of azole-resistant Candida albicans by mutation of sterol 14-demethylase P450.},
  author={Kentaro Asai and Noboru Tsuchimori and Kenji Okonogi and John R Perfect and Osamu Gotoh and Yasuo Yoshida},
  journal={Antimicrobial agents and chemotherapy},
  year={1999},
  volume={43 5},
  pages={1163-9}
}
The sterol 14-demethylase P450 (CYP51) of a fluconazole-resistant isolate of Candida albicans, DUMC136, showed reduced susceptibility to this azole but with little change in its catalytic activity. Twelve nucleotide substitutions, resulting in four amino acid changes, were identified in the DUMC136 CYP51 gene in comparison with a reported CYP51 sequence from a wild-type, fluconazole-susceptible C. albicans strain. Seven of these substitutions, including all of those causing amino acid changes… CONTINUE READING