Formation of an Fe(III)-tyrosinate complex during biomineralization of H-subunit ferritin.

@article{Waldo1993FormationOA,
  title={Formation of an Fe(III)-tyrosinate complex during biomineralization of H-subunit ferritin.},
  author={Geoff S Waldo and Jianhua Ling and Joann Sanders-Loehr and Elizabeth C. Theil},
  journal={Science},
  year={1993},
  volume={259 5096},
  pages={796-8}
}
An iron(III)-tyrosinate complex was identified in ferritin by ultraviolet-visible and resonance Raman spectroscopies. Previously, a specific amino acid side chain coordinated to iron in ferritin was not known. Ferritin protein was overexpressed in Escherichia coli from complementary DNA sequences of bullfrog red cell ferritin. The purple iron(III)-tyrosinate intermediate that formed during the first stages of iron uptake was replaced by the amber multinuclear iron(III)-oxo complexes of fully… CONTINUE READING