Formation of adenosine 5'-tetraphosphate from the acyl phosphate intermediate: a difference between the MurC and MurD synthetases of Escherichia coli.

@article{Bouhss1999FormationOA,
  title={Formation of adenosine 5'-tetraphosphate from the acyl phosphate intermediate: a difference between the MurC and MurD synthetases of Escherichia coli.},
  author={Ahmed Bouhss and S{\'e}bastien Dementin and Jean van Heijenoort and Claudine Parquet and Didier Blanot},
  journal={FEBS letters},
  year={1999},
  volume={453 1-2},
  pages={15-9}
}
The mechanism of the Mur synthetases of peptidoglycan biosynthesis is thought to involve in each case the successive formation of an acyl phosphate and a tetrahedral intermediate. The existence of the acyl phosphates for the MurC and MurD enzymes from Escherichia coli was firmly established by their in situ reduction by sodium borohydride followed by acid… CONTINUE READING