Formation of a quaternary N-glucuronide of amitriptyline in human liver microsomes.

@article{DahlPuustinen1987FormationOA,
  title={Formation of a quaternary N-glucuronide of amitriptyline in human liver microsomes.},
  author={M L Dahl-Puustinen and Leif Bertilsson},
  journal={Pharmacology & toxicology},
  year={1987},
  volume={61 5},
  pages={342-6}
}
Amitriptyline N-glucuronide was isolated from urine of a patient treated with therapeutic doses of amitriptyline. The glucuronide was hydrolyzed by hot alkaline treatment and, to a lesser degree, by treatment with beta-glucuronidase. A method for the direct measurement of amitriptyline glucuronide by HPLC was developed. Human liver microsomes were shown to glucuronidate amitriptyline in the presence of UDPGA, and the activity varied 7-fold among microsomes from 13 different human livers. The… CONTINUE READING