Formation of SRP-like particle induces a conformational change in E. coli 4.5S RNA.

@article{Lentzen1994FormationOS,
  title={Formation of SRP-like particle induces a conformational change in E. coli 4.5S RNA.},
  author={Georg Lentzen and Bernhard Dobberstein and Wolfgang Wintermeyer},
  journal={FEBS letters},
  year={1994},
  volume={348 3},
  pages={
          233-8
        }
}
E. coli P48 protein is homologous to the SRP54 component of the eukaryotic signal recognition particle. In vivo, P48 is associated with 4.5S RNA which shares a homology with eukaryotic SRP RNA. To study the interaction between P48 and 4.5S RNA in vitro, we used 4.5S RNA with fluorescein coupled to the 3'-terminal ribose. Upon binding of P48, the fluorescent 4.5S RNA shows a substantial decrease in fluorescence. Fluorescence quenching as well as anisotropy measurements reveal that the effect is… CONTINUE READING

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