Formation of Fenestrae in Murine Liver Sinusoids Depends on Plasmalemma Vesicle-Associated Protein and Is Required for Lipoprotein Passage

@article{Herrnberger2014FormationOF,
  title={Formation of Fenestrae in Murine Liver Sinusoids Depends on Plasmalemma Vesicle-Associated Protein and Is Required for Lipoprotein Passage},
  author={Leonie Herrnberger and R. Hennig and W. Kremer and C. Hellerbrand and A. Goepferich and H. Kalbitzer and E. Tamm},
  journal={PLoS ONE},
  year={2014},
  volume={9}
}
Liver sinusoidal endothelial cells (LSEC) are characterized by the presence of fenestrations that are not bridged by a diaphragm. The molecular mechanisms that control the formation of the fenestrations are largely unclear. Here we report that mice, which are deficient in plasmalemma vesicle-associated protein (PLVAP), develop a distinct phenotype that is caused by the lack of sinusoidal fenestrations. Fenestrations with a diaphragm were not observed in mouse LSEC at three weeks of age, but… Expand
Fenestral diaphragms and PLVAP associations in liver sinusoidal endothelial cells are developmentally regulated
TLDR
The data show that PLVAP can be expressed on endothelial cells without diaphragms, contradict the prevailing concept that biogenesis of fenestrae would bePLVAP-dependent, and reveal previously unknown PLV AP-dependent molecular complexes in LSEC during angiogenesis. Expand
Cavin1 Deficiency Causes Disorder of Hepatic Glycogen Metabolism and Neonatal Death by Impacting Fenestrations in Liver Sinusoidal Endothelial Cells
  • Zhuang Wei, Jigang Lei, +9 authors Shangyu Hong
  • Medicine
  • Advanced science
  • 2020
TLDR
A novel function of Cavin1 is revealed on fenestrae formation in LSECs and liver glycogen metabolism, which provide an explanation for the neonatal death of CavIn1 null mice and a potential mechanism for metabolic disorders in patients with Cavin 1 mutation. Expand
Bone Morphogenetic Protein 9 Is a Paracrine Factor Controlling Liver Sinusoidal Endothelial Cell Fenestration and Protecting Against Hepatic Fibrosis
TLDR
Observations show that BMP9 is a key paracrine regulator of liver homeostasis, controlling LSEC fenestration and protecting against perivascular hepatic fibrosis. Expand
The potential role of liver sinusoidal endothelial cells in drug-induced liver injury
Liver sinusoidal endothelial cells (LSEC) constitute a unique population of endothelial cells with specialised liver-specific morphologic features and functions. LSEC are the only endothelial cellsExpand
LSEC Fenestrae Are Preserved Despite Pro-inflammatory Phenotype of Liver Sinusoidal Endothelial Cells in Mice on High Fat Diet
TLDR
In the early and late phases of NAFLD, despite metabolic and pro-inflammatory burden linked to HFD, LSEC fenestrae and bioenergetics are functionally preserved and suggest prominent adaptive capacity of LSECs that might mitigateNAFLD progression. Expand
Transcriptomic Analysis of Hepatic Cells in Multicellular Organotypic Liver Models
TLDR
The first transcriptomic study of liver sinusoidal endothelial cells (LSECs) and Kupffer cells (KCs) cultured with hepatocytes is reported, and it is demonstrated that KCs in 3D liver models display expression patterns consistent with an anti-inflammatory phenotype when compared to monocultures. Expand
Angiodiversity and organotypic functions of sinusoidal endothelial cells
TLDR
The identification and functional analysis of LSEC-derived angiocrine signals, which control liver homeostasis and disease pathogenesis in an instructive manner, marks a major change of paradigm in the understanding of liver function in health and disease. Expand
Liver sinusoidal endothelial cells: Physiology and role in liver diseases.
TLDR
This review provides an overview of the strategies available for a specific targeting of LSECs and presents a detailed analysis of the technical aspects relevant for LSEC analysis including the markers these cells express, the available cell lines and the transgenic mouse models. Expand
Role of liver sinusoidal endothelial cells in liver diseases
TLDR
The main functions and phenotypic dysregulations of LSECs in liver diseases, specifically in the context of acute injury, drug-induced liver injury, chronic liver disease and hepatocellular carcinoma are described and an update of the role of L SECs as therapeutic targets is provided. Expand
Kidney-resident macrophages promote a proangiogenic environment in the normal and chronically ischemic mouse kidney
Renal artery stenosis (RAS) caused by narrowing of arteries is characterized by microvascular damage. Macrophages are implicated in repair and injury, but the specific populations responsible forExpand
...
1
2
3
4
...

References

SHOWING 1-10 OF 58 REFERENCES
Lack of endothelial diaphragms in fenestrae and caveolae of mutant Plvap-deficient mice
Plasmalemmal vesicle-associated protein (PLVAP, PV-1) is specifically expressed in endothelial cells in which it localizes to diaphragms of fenestrae, caveolae, and transendothelial channels. ToExpand
An in vitro assay reveals a role for the diaphragm protein PV-1 in endothelial fenestra morphogenesis
TLDR
An in vitro assay is presented in which rapid and abundant fenestra induction enables a detailed study of their biogenesis and it is shown that actin microfilament remodeling is part of fenESTra biogenesis in this model. Expand
The role of plasmalemma vesicle-associated protein (PLVAP) in endothelial cells of Schlemm's canal and ocular capillaries.
TLDR
Diaphragms were absent in fenestrae of endothelial cells in the capillaries of the ciliary processes or the choriocapillaris, findings which were associated with a substantial decrease in the number of fenESTrae. Expand
The size of endothelial fenestrae in human liver sinusoids: implications for hepatocyte-directed gene transfer
TLDR
The small diameter of fenestrae in humans is likely to be a major obstacle for hepatocyte transduction by adenoviral vectors. Expand
PV-1 is a component of the fenestral and stomatal diaphragms in fenestrated endothelia.
  • R. Stan, M. Kubitza, G. Palade
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1999
TLDR
These findings define the only antigen to be localized thus far in fenestral diaphragms and show that the stomatal diaphagms of caveolae and transendothelial channels and the fenESTral diphragms might be biochemically related, in addition to being morphologically similar structures. Expand
The diaphragms of fenestrated endothelia: gatekeepers of vascular permeability and blood composition.
TLDR
Genetic evidence is provided for the critical role of the diaphragms in fenestrated capillaries in the maintenance of blood composition by deletion of the PV1-encoding Plvap gene in mice. Expand
Role of extracellular matrix in regulating fenestrations of sinusoidal endothelial cells isolated from normal rat liver
TLDR
Fenestral density and porosity were significantly enhanced, however, when endothelial cells were cultured on the basement‐membrane side of human amnion, suggesting that support of endothelial fenestrations requires a complex matrix. Expand
The Relationship between Fenestrations, Sieve Plates and Rafts in Liver Sinusoidal Endothelial Cells
TLDR
The results are consistent with a sieve-raft interaction, where fenestrations form in non-raft lipid-disordered regions of endothelial cells once the membrane-stabilizing effects of actin cytoskeleton and membrane rafts are diminished. Expand
A novel structure involved in the formation of liver endothelial cell fenestrae revealed by using the actin inhibitor misakinolide.
TLDR
Using the new actin inhibitor misakinolide, this work observed a new structure that appears to serve as a fenestrae-forming center in hepatic endothelial cells. Expand
Microfilament‐disrupting agent latrunculin A induces and increased number of fenestrae in rat liver sinusoidal endothelial cells: Comparison with cytochalasin B
TLDR
Two agents that alter the state of actin organization in LECs, albeit by different mechanisms, cause the doubling of the number of fenestrae within 10 to 30 minutes, which indicates that thestate of assembly of the actin cytoskeleton is important in the numerical dynamics of LEC fenESTrae and that the act in cytos skeleton is probably the main mechanical regulator for sieving between the sinusoidal blood and the parenchymal cells. Expand
...
1
2
3
4
5
...