Formation of DNA adducts in human skin maintained in short-term organ culture and treated with coal-tar, creosote or bitumen.

@article{Schoket1988FormationOD,
  title={Formation of DNA adducts in human skin maintained in short-term organ culture and treated with coal-tar, creosote or bitumen.},
  author={Bernadette Schoket and A. J. H. Hewer and Philip L Grover and David H Phillips},
  journal={International journal of cancer},
  year={1988},
  volume={42 4},
  pages={622-6}
}
Adult and foetal human skin samples maintained in short-term organ culture were treated topically with solutions of coal-tar, creosote or bitumen, then DNA was isolated and analysed by 32P-post-labelling for the presence of aromatic DNA adducts. Autoradiographs of the 32P-labelled adducts resolved on polyethyleneimine-cellulose tlc sheets revealed a band of radioactivity indicative of the formation of adducts by a large number of components in these complex carcinogenic mixtures. Single doses… CONTINUE READING

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