Formation of AP-3 transport intermediates requires Vps41 function

  title={Formation of AP-3 transport intermediates requires Vps41 function},
  author={Peter Rehling and Tamara Darsow and David J. Katzmann and Scott D Emr},
  journal={Nature Cell Biology},
Transport of a subset of membrane proteins to the yeast vacuole requires the function of the AP-3 adaptor protein complex. To define the molecular requirements of vesicular transport in this pathway, we used a biochemical approach to analyse the formation and content of the AP-3 transport intermediate. A vam3tsf (vacuolar t-SNARE) mutant blocks vesicle docking and fusion with the vacuole and causes the accumulation of 50–130-nanometre membrane vesicles, which we isolated and showed by… CONTINUE READING
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