Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases

@article{Murayama2008FormationAB,
  title={Formation and branch migration of Holliday junctions mediated by eukaryotic recombinases},
  author={Yasuto Murayama and Yu Kurokawa and Kouta Mayanagi and Hiroshi Iwasaki},
  journal={Nature},
  year={2008},
  volume={451},
  pages={1018-1021}
}
Holliday junctions (HJs) are key intermediates in homologous recombination and are especially important for the production of crossover recombinants. Bacterial RecA family proteins promote the formation and branch migration of HJs in vitro by catalysing a reciprocal DNA-strand exchange reaction between two duplex DNA molecules, one of which contains a single-stranded DNA region that is essential for initial nucleoprotein filament formation. This activity has been reported only for prokaryotic… Expand
An in vitro assay for monitoring the formation and branch migration of holliday junctions mediated by a eukaryotic recombinase.
TLDR
In vitro analyses using purified recombination proteins and model DNA substrates provide a mechanistic insight into the DNA strand exchange reaction, including the steps leading to the formation and branch migration of Holliday junctions. Expand
The fission yeast meiosis-specific Dmc1 recombinase mediates formation and branch migration of Holliday junctions by preferentially promoting strand exchange in a direction opposite to that of Rad51.
TLDR
It is shown that Dmc1 from fission yeast has a similar activity, which requires ATP hydrolysis and is independent of an absolute requirement for the Swi5-Sfr1 complex, suggesting that strand exchange reactions between duplex-duplex and single-duple DNAs are mechanistically different. Expand
The RecA/RAD51 protein drives migration of Holliday junctions via polymerization on DNA
TLDR
It is demonstrated that cycles of RecA/Rad51 polymerization and dissociation coupled with ATP hydrolysis drives the BM of HJs, a key intermediate in homologous recombination, DNA repair, and replication. Expand
Polarity and Bypass of DNA Heterology during Branch Migration of Holliday Junctions by Human RAD54, BLM, and RECQ1 Proteins*
TLDR
BM is defined as a mechanistically distinct activity of DNA translocating proteins, which may serve an important function in DNA repair and recombination. Expand
Rad54, the motor of homologous recombination.
TLDR
The properties of Rad54 are described, an important and versatile HR protein that is evolutionarily conserved in eukaryotes and a motor protein that translocates along dsDNA and performs several important functions in HR. Expand
Rad51 and Dmc1 Recombinases
In eukaryotes, Rad51 and its meiosis-specific homologue Dmc1 (disrupted meiotic cDNA1) are key proteins of the homologous recombination (HR) pathway, which is responsible for the error-free repair ofExpand
Reconstituting the 4-Strand DNA Strand Exchange.
TLDR
The presented methodology may be used for reconstitution of the medial-to-late stages of homologous recombination in vitro as well as for investigation of the mechanisms of branch migration by helicase-like proteins, e.g., Rad54, BLM, or RecQ1. Expand
Heterology tolerance and recognition of mismatched base pairs by human Rad51 protein.
TLDR
AnhRad51 mutant protein (hRad 51K133R), deficient in ATP hydrolysis, showed greater heterology tolerance to both types of mismatch base pairing, suggesting that ATPase activity may be important for maintenance of high fidelity homologous recombination DNA repair. Expand
From strand exchange to branch migration; bypassing of non-homologous sequences by human Rad51 and Rad54.
TLDR
It is proposed that the main roles of hRad51 in homologous recombination is to initiate the homology recognition and strand-exchange steps and those of h Rad54 are to promote efficient branch migration, bypass potential mismatches and facilitate long-range strand exchanges through branch migration of Holliday junctions. Expand
Brh2 promotes a template-switching reaction enabling recombinational bypass of lesions during DNA synthesis.
TLDR
It is proposed that template switching promoted by Brh2 provides a mechanism for recombination-mediated bypass of lesions blocking synthesis during DNA replication. Expand
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