Formate dehydrogenase from methylotrophic bacterium Pseudomonas sp. 101: gene cloning and expression in Escherichia coli.

@article{Tishkov1993FormateDF,
  title={Formate dehydrogenase from methylotrophic bacterium Pseudomonas sp. 101: gene cloning and expression in Escherichia coli.},
  author={Vladimir I. Tishkov and Andrey G Galkin and George N Marchenko and Yuri D. Tsygankov and A. M. Egorov},
  journal={Biotechnology and applied biochemistry},
  year={1993},
  volume={18 ( Pt 2)},
  pages={201-7}
}
Using two degenerate 20- and 23-mer oligonucleotide probes, the gene encoding NAD(+)-dependent formate dehydrogenase (FDH) (EC 1.2.1.2) was shown to lie within a 3.5 kb PstI fragment of the chromosomal DNA of methylotrophic bacterium Pseudomonas sp. 101. A phasmid library was prepared in the lambda pSL5 vector with partial EcoRI-digested DNA from Pseudomonas sp. 101. The 12 clones selected contained three types of phasmid: lambda pFDH1, lambda pFDH2 and lambda pFDH3 (with inserts of 15, 17.6… CONTINUE READING