Formate dehydrogenase from Methylosinus trichosporium OB3b. Purification and spectroscopic characterization of the cofactors.

@article{Jollie1991FormateDF,
  title={Formate dehydrogenase from Methylosinus trichosporium OB3b. Purification and spectroscopic characterization of the cofactors.},
  author={David R. Jollie and John D Lipscomb},
  journal={The Journal of biological chemistry},
  year={1991},
  volume={266 32},
  pages={21853-63}
}
NAD(+)-coupled formate dehydrogenase has been purified to near-homogeneity from the obligate methanotroph Methylosinus trichosporium OB3b. The inclusion of stabilizing reagents in the purification buffers has resulted in a 3-fold increase in specific activity (98 microM/min/mg; turnover number 600 s-1) and as much as a 25-fold increase in yield over… CONTINUE READING