Forensic application of a rapid and quantitative DNA sex test by amplification of the X-Y homologous gene amelogenin

@article{Mannucci2005ForensicAO,
  title={Forensic application of a rapid and quantitative DNA sex test by amplification of the X-Y homologous gene amelogenin},
  author={Armando Mannucci and Kevin M. Sullivan and Pavel L. Ivanov and Peter Gill},
  journal={International Journal of Legal Medicine},
  year={2005},
  volume={106},
  pages={190-193}
}
SummaryGender identification of forensic samples was determined by amplifying a segment of the X-Y homologous gene amelogenin. Using a single pair of primers spanning part of the first intron, 106 by and 112 by PCR products were generated from the X and Y homologues respectively, which were then resolved by agarose gel electrophoresis. This test enabled as little as 20 pg of DNA from severely degraded bones to be amplified and typed in a single tube reaction. Furthermore, using dye-labelled… 

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A polymerase chain reaction (PCR)-based multiplex approach to improve the certainty of molecular sex identification on archaeological skeletal material by coamplifying amelogenin, two X-chromosomal short tandem repeats (STRs), and two Y-specific STRs, which was successfully applied to 3,000-year-old bone material.
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