Corpus ID: 11930405

Forensic Detection of Semen II

  title={Forensic Detection of Semen II},
  author={L. Laux and A. J. Tambasco and E. Benzinger},
Prostatic specific antigen (PSA) or p30, was first described in 1971 by Hara, (1) which they called -seminoprotein. Li and Beling (2) described what most likely was the same protein that they called E1 in 1973. A thorough biochemical analysis of a protein isolated from semen utilizing electrophoretic methods was made by Sensabaugh (3) in 1978. He termed the protein p30 as its molecular weight was approximately 30 kdaltons. Graves, (4) studied the protein extensively and the work… Expand
The results showed the lack of cross reactivity between PSA in semen from ram, jack and human, and the SERATEC® PSA Semiquant test is human-specific and not appropriate for detection of prostate-specific antigen in ether ram or jack semen. Expand
The validity of rapid test to detect prostate-specific antigen (PSA) in seminal fluid
PSA Rapid Test is very specific and sensitive to detect PSA in seminal fluid and therefore, this device is suggested for forensic use in sexual assault cases. Expand
An autopsy report case of rape victim by the application of PSA test kit as a new innovation for sexual assault investigation in Thailand.
The present report suggests the PSA test kit in addition to conventional sperm smear and acid phosphatase test for sexual assault investigation in Thailand. Expand


Isolation and characterization of two specific antigens of human seminal plasma.
2 specific antigens of human seminal plasma (HSP) were isolated and the antigen is specific to HSP is highly immunogenic and is not likely to originate in the prostate. Expand
Evaluation of prostate-specific antigen (PSA) membrane test assays for the forensic identification of seminal fluid.
Three immunochromatographic PSA membrane test assays are evaluated by analyzing semen stains stored at room temperature for up to 30 years, post-coital vaginal swabs taken at different time after intercourse, semen-free vaginalSwabs, and various female and male body fluids, including urine to demonstrate that PSA membranes test Assays offer the same sensitivity as ELISA-based tests and provide a rapid approach for the forensic identification of seminal fluid. Expand
Isolation and characterization of a semen-specific protein from human seminal plasma: a potential new marker for semen identification.
The genetic basis of specificity of the acid phosphatase test is in question; the quantitative test can only be based on the extraordinarily high level of acidosphatase activity in semen; the low levels of activity often found in postcoital vaginal washings are thus equivocal with respect to the question of semen detection. Expand
Measurement of prostate-specific antigen and human glandular kallikrein 2 in different body fluids.
Measurements show that in addition to the previously known secretion in seminal plasma, hK2 is secreted in amniotic fluid, breast milk, and saliva, suggesting that the two proteases might form a functional unit but not always as demonstrated by the sole presence of h kallikrein 2 in saliva. Expand
Validation of the use of a commercially available kit for the identification of prostate specific antigen (PSA) in semen stains.
The results indicate that the Tandem-E PSA Immunoenzymetric Assay can be used to identify the presence of semen in forensically significant specimens. Expand
Use of the "SMITEST" PSA card to identify the presence of prostate-specific antigen in semen and male urine.
The PSA card is useful for identification of PSA in both semen and adult male urine, and the immunoreactive line showing PSA activity became weak after storage, it was still detectable, but faint, after 3 years. Expand
Nonprostatic sources of prostate-specific antigen.
Measuring PSA in breast cancer cytosol, breast-nipple aspirate fluid, and female serum may have potential clinical utilities, including breast cancer prognosis, breast cancer risk assessment, and evaluation of androgen excess. Expand
Detection of nonprostatic PSA in serum and nonserum samples from women
It is concluded that PSA can no longer be considered a specific prostate tissue marker; since the concentrations detected in women's serum are ultralow, this finding appears to interfere minimally with its value as a tumoral marker. Expand
Prostate-specific antigen immunoreactivity in amniotic fluid.
It is thought PSA may serve as a growth factor regulator in cancer and in normal fetal development during pregnancy, and its concentration increases with increasing gestational age from 11 to 21 weeks, decreasing at delivery. Expand
Immunoreactive prostatic specific antigen in male periurethral glands.
Evidence disproves the fact that PSA and PAP are organ specific as previously described, and more than likely any tissue of cloacal origin has potential for staining positive for prostatic specific antigen and prostatic acid phosphatase. Expand