Folylpolyglutamate synthase from higher plants.

  title={Folylpolyglutamate synthase from higher plants.},
  author={Helena Cecile. Imeson and Edwin A. Cossins},
  journal={Methods in enzymology},
Publisher Summary Although folylpolyglutamate synthase activity (FPGS) has been reported from a number of different plant species, only that from pea seeds has been purified and characterized. This chapter presents folylpolyglutamate synthase from higher plants. The assay is based on the incorporation of tritiated L -glutamate into folylpolyglutamates with tetrahydrofolate monoglutamate serving as the folate substrate. The isolation of polyglutamate products involves an ion-exchange procedure… CONTINUE READING