Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase. Cloning and high expression of the Escherichia coli folC gene and purification and properties of the gene product.

@article{Bognar1985FolylpolygammaglutamateSS,
  title={Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase. Cloning and high expression of the Escherichia coli folC gene and purification and properties of the gene product.},
  author={Andrew L. Bognar and C B Osborne and Barry Shane and S C Singer and R Ferone},
  journal={The Journal of biological chemistry},
  year={1985},
  volume={260 9},
  pages={5625-30}
}
The Escherichia coli gene for folylpolyglutamate synthetase-dihydrofolate synthetase was localized to plasmids pLC22-45, 24-31, and 28-44 of the Clarke-Carbon E. coli colony bank (Clarke, L., and Carbon, J. (1976) Cell 9, 91-99) by screening the bank by replica mating with an E. coli folC mutant. The folC gene was subcloned from pLC22-45 and inserted into a high copy number plasmid containing the lambda replication control region under the control of the temperature-sensitive cI857 repressor… CONTINUE READING

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References

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Showing 1-2 of 2 references

Anal . Biochem . 8 6 , 142 - 146 Biochem . 39 , 209 - 228 chemistry 10 , 2606 - 2617 ( 1983 )

  • F. C. Neidhardt, V. Vaughn, H. Kleinkauf, H. Dohren, P. F. Nixon, R. L. Blakley
  • Microbiol . Reu .
  • 1978

Experiments i n Molecular Genetics, p

  • W S., G. Milman
  • J. Biol. Ckm
  • 1976

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