Follicular viability and morphology of sheep ovaries after exposure to cryoprotectant and cryopreservation with different freezing protocols.

@article{Demirci2001FollicularVA,
  title={Follicular viability and morphology of sheep ovaries after exposure to cryoprotectant and cryopreservation with different freezing protocols.},
  author={Banu Demirci and Jacqueline Lornage and Bruno S. Salle and Lucien Frappart and Michel Franck and Jean François Gu{\'e}rin},
  journal={Fertility and sterility},
  year={2001},
  volume={75 4},
  pages={754-62}
}
OBJECTIVE To test the toxicity of cryoprotectant in sheep ovarian tissue and to determine optimal conditions for freezing hemiovary cortex. DESIGN Small follicles (<60 microm in diameter) were isolated enzymatically for viability testing. Dead and live follicles were identified by using trypan blue staining, and follicle morphology was examined histologically. SETTING Centre hospitalo-universitaire de Biologie de la Reproduction, Hôpital Edouard Herriot, Lyon, France. ANIMAL(S) Lambs 5 to… CONTINUE READING
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