Folding of firefly luciferase during translation in a cell-free system.

@article{Kolb1994FoldingOF,
  title={Folding of firefly luciferase during translation in a cell-free system.},
  author={Vyacheslav A. Kolb and Eugene V Makeyev and Alexander S. Spirin},
  journal={The EMBO journal},
  year={1994},
  volume={13 15},
  pages={3631-7}
}
In vitro synthesis of firefly luciferase and its folding into an enzymatically active conformation were studied in a wheat germ cell-free translation system. A novel method is described by which the enzymatic activity of newly synthesized luciferase can be monitored continuously in the cell-free system while this protein is being translated from its mRNA. It is shown that ribosome-bound polypeptide chains have no detectable enzymatic activity, but that this activity appears within a few seconds… CONTINUE READING