Folding and assembly of lambda Cro repressor dimers are kinetically limited by proline isomerization.

@article{Satumba2002FoldingAA,
  title={Folding and assembly of lambda Cro repressor dimers are kinetically limited by proline isomerization.},
  author={W. John Satumba and Michael C. Mossing},
  journal={Biochemistry},
  year={2002},
  volume={41 48},
  pages={14216-24}
}
Cro binds to operator sites in lambda DNA as a dimer. Dimerization of this small repressor protein is weak, however, and proline residues in the dimer interface suggest that folding and assembly of active repressors may be complex. Cro and selected variants have been studied by circular dichroism and fluorescence. Fluorescent probes include a unique tryptophan residue in the dimer interface and extrinsic resonance energy transfer probes that monitor dimerization. Both folding and unfolding are… CONTINUE READING