Folding Optimization In Vivo Uncovers New Chaperones.

@article{Lennon2015FoldingOI,
  title={Folding Optimization In Vivo Uncovers New Chaperones.},
  author={Christopher William Lennon and Maike Thamsen and Elias T Friman and Austin Cacciaglia and Veronika Sachsenhauser and Frieda A Sorgenfrei and Milena A Wasik and James C. A. Bardwell},
  journal={Journal of molecular biology},
  year={2015},
  volume={427 18},
  pages={
          2983-94
        }
}
By employing a genetic selection that forces the cell to fold an unstable, aggregation-prone test protein in order to survive, we have generated bacterial strains with enhanced periplasmic folding capacity. These strains enhance the soluble steady-state level of the test protein. Most of the bacterial variants we isolated were found to overexpress one or more periplasmic proteins including OsmY, Ivy, DppA, OppA, and HdeB. Of these proteins, only HdeB has convincingly been previously shown to… CONTINUE READING
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