Folate transport and binding by cultured human proximal tubule cells.

@article{McMartin1992FolateTA,
  title={Folate transport and binding by cultured human proximal tubule cells.},
  author={Kenneth E. McMartin and Khandoker M. Morshed and Debra J. Hazen-Martin and Donald A Sens},
  journal={The American journal of physiology},
  year={1992},
  volume={263 5 Pt 2},
  pages={F841-8}
}
Because mechanisms for the renal regulation of folate excretion are poorly understood, a cell culture system representative of the human proximal tubule (HPT) was used for studies of renal folate transport. After confluent cultures of HPT cells were incubated with 3H-labeled folic acid (PteGlu), binding to the apical membrane was determined by an acid removal procedure, and transport was subsequently measured in solubilized cells. Although PteGlu binding was almost all specific (suppressed by… CONTINUE READING