Folate fortification of rice by metabolic engineering

  title={Folate fortification of rice by metabolic engineering},
  author={Sergei Storozhenko and Veerle De Brouwer and Maarten Volckaert and Oscar U. Navarrete and Dieter Blancquaert and G Zhang and Willy Lambert and Dominique Van Der Straeten},
  journal={Nature Biotechnology},
Rice, the world's major staple crop, is a poor source of essential micronutrients, including folates (vitamin B9). We report folate biofortification of rice seeds achieved by overexpressing two Arabidopsis thaliana genes of the pterin and para-aminobenzoate branches of the folate biosynthetic pathway from a single locus. We obtained a maximal enhancement as high as 100 times above wild type, with 100 g of polished raw grains containing up to four times the adult daily folate requirement.