Corpus ID: 31620007

Folate depletion induced by methotrexate affects methionine synthase activity and its susceptibility to inactivation by nitrous oxide.

@article{Fiskerstrand1997FolateDI,
  title={Folate depletion induced by methotrexate affects methionine synthase activity and its susceptibility to inactivation by nitrous oxide.},
  author={T. Fiskerstrand and P. Ueland and H. Refsum},
  journal={The Journal of pharmacology and experimental therapeutics},
  year={1997},
  volume={282 3},
  pages={
          1305-11
        }
}

T. Fiskerstrand, P. Ueland, H. Refsum

Published 1997

Chemistry, Medicine

The Journal of pharmacology and experimental therapeutics

We compared the effects of methotrexate (MTX) and nitrous oxide on the methionine (Met) synthase system in two variants of a human glioma cell line. The cells were protected from cytotoxic effect of MTX by adding thymidine and hypoxanthine to the cell culture medium. MTX (0-1 microM) was associated with a dose- and time-dependent reduction in 5-methyltetrahydrofolate (5-methyl-THF) in both cell lines. Already after 3 hr of exposure, 5-methyl-THF was reduced by 50% and after additional 48 hr… CONTINUE READING

View on PubMed

folk.uib.no

Save to Library

Create Alert

Cite

Launch Research Feed