Corpus ID: 31620007

Folate depletion induced by methotrexate affects methionine synthase activity and its susceptibility to inactivation by nitrous oxide.

  title={Folate depletion induced by methotrexate affects methionine synthase activity and its susceptibility to inactivation by nitrous oxide.},
  author={T. Fiskerstrand and P. Ueland and H. Refsum},
  journal={The Journal of pharmacology and experimental therapeutics},
  volume={282 3},
  • T. Fiskerstrand, P. Ueland, H. Refsum
  • Published 1997
  • Chemistry, Medicine
  • The Journal of pharmacology and experimental therapeutics
  • We compared the effects of methotrexate (MTX) and nitrous oxide on the methionine (Met) synthase system in two variants of a human glioma cell line. The cells were protected from cytotoxic effect of MTX by adding thymidine and hypoxanthine to the cell culture medium. MTX (0-1 microM) was associated with a dose- and time-dependent reduction in 5-methyltetrahydrofolate (5-methyl-THF) in both cell lines. Already after 3 hr of exposure, 5-methyl-THF was reduced by 50% and after additional 48 hr… CONTINUE READING
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