Focused genetic recombination of bacteriophage t4 initiated by double-strand breaks.

@article{Shcherbakov2002FocusedGR,
  title={Focused genetic recombination of bacteriophage t4 initiated by double-strand breaks.},
  author={Victor Prokhorovich Shcherbakov and Igor E. Granovsky and Lidiya Plugina and Tamara Shcherbakova and Svetlana Sizova and Konstantin I. Pyatkov and M. G. Shlyapnikov and Olga Shubina},
  journal={Genetics},
  year={2002},
  volume={162 2},
  pages={543-56}
}
A model system for studying double-strand-break (DSB)-induced genetic recombination in vivo based on the ets1 segCDelta strain of bacteriophage T4 was developed. The ets1, a 66-bp DNA fragment of phage T2L containing the cleavage site for the T4 SegC site-specific endonuclease, was inserted into the proximal part of the T4 rIIB gene. Under segC(+) conditions, the ets1 behaves as a recombination hotspot. Crosses of the ets1 against rII markers located to the left and to the right of ets1 gave… CONTINUE READING