Fluoroquinolone-gyrase-DNA complexes: two modes of drug binding.

  title={Fluoroquinolone-gyrase-DNA complexes: two modes of drug binding.},
  author={Arkady Mustaev and Muhammad Usama Malik and Xilin Zhao and Natalia Kurepina and Gan Luan and Lisa M. Oppegard and Hiroshi Hiasa and Kevin Randall Marks and Robert J Kerns and James M Berger and Karl Drlica},
  journal={The Journal of biological chemistry},
  volume={289 18},
DNA gyrase and topoisomerase IV control bacterial DNA topology by breaking DNA, passing duplex DNA through the break, and then resealing the break. This process is subject to reversible corruption by fluoroquinolones, antibacterials that form drug-enzyme-DNA complexes in which the DNA is broken. The complexes, called cleaved complexes because of the presence of DNA breaks, have been crystallized and found to have the fluoroquinolone C-7 ring system facing the GyrB/ParE subunits. As expected… CONTINUE READING