Fluorophores for live cell imaging of AGT fusion proteins across the visible spectrum.

@article{Keppler2006FluorophoresFL,
  title={Fluorophores for live cell imaging of AGT fusion proteins across the visible spectrum.},
  author={Antje Keppler and Claudio Arrivoli and Lucia Sironi and Jan Ellenberg},
  journal={BioTechniques},
  year={2006},
  volume={41 2},
  pages={
          167-70, 172, 174-5
        }
}
O6-alkylguanine-DNA alkyltransferase (AGT) fusion proteins can be specifically and covalently labeled with fluorescent O6-benzylguanine (O6-BG) derivatives for multicolor live cell imaging approaches. Here, we characterize several new BG fluorophores suitable for in vivo AGT labeling that display fluorescence emission maxima covering the visible spectrum from 472 to 673 nm, thereby extending the spectral limits set by fluorescent proteins. We show that the photostability of the cell-permeable… CONTINUE READING
26 Citations
26 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 26 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 26 references

Similar Papers

Loading similar papers…