Fluorophore-conjugated iron oxide nanoparticle labeling and analysis of engrafting human hematopoietic stem cells.

@article{Maxwell2008FluorophoreconjugatedIO,
  title={Fluorophore-conjugated iron oxide nanoparticle labeling and analysis of engrafting human hematopoietic stem cells.},
  author={Dustin J Maxwell and Jesper Bonde and David A Hess and Sarah A Hohm and R. I. Lahey and Ping Zhou and Michael H. Creer and David R. Piwnica-Worms and Jan A Nolta},
  journal={Stem cells},
  year={2008},
  volume={26 2},
  pages={517-24}
}
The use of nanometer-sized iron oxide particles combined with molecular imaging techniques enables dynamic studies of homing and trafficking of human hematopoietic stem cells (HSC). Identifying clinically applicable strategies for loading nanoparticles into primitive HSC requires strictly defined culture conditions to maintain viability without inducing terminal differentiation. In the current study, fluorescent molecules were covalently linked to dextran-coated iron oxide nanoparticles… CONTINUE READING