Fluorometrische Bestimmung von “aktiviertem” Cyclophosphamid und Ifosfamid im Blut

@article{Voelcker2004FluorometrischeBV,
  title={Fluorometrische Bestimmung von “aktiviertem” Cyclophosphamid und Ifosfamid im Blut},
  author={Georg Voelcker and R. Haeglsperger and H. J. Hohorst},
  journal={Journal of Cancer Research and Clinical Oncology},
  year={2004},
  volume={93},
  pages={233-240}
}
“Activated” N-(2-Chloroethyl)amido-oxazaphosphorines like 4-hydroxycyclophosphamide, 4-hydroperoxycyclophosphamide, 4-hydroxyifosfamide, and 4-hydroperoxyifosfamide can be determined fluorometrically by condensation of liberated acrolein with m-aminophenol yielding 7-hydroxychinolin. The method permits determination of 10-10 mol and is specific for “activated” N-(2-Chloroethyl)amido-oxazaphosphorine metabolites which liberate acrolein under conditions of the test. Neither cyclophosphamide nor… CONTINUE READING