Fluorometric measurements of riboflavin and its natural derivatives in small quantities of blood serum and cells.

  title={Fluorometric measurements of riboflavin and its natural derivatives in small quantities of blood serum and cells.},
  author={Helen B. Burch and Otto A. Bessey and Oliver H. Lowry},
  journal={The Journal of biological chemistry},
  volume={175 1},

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Assessment of status riboflavin nutriture by assay of erythrocyte glutathione reductase activity.

The status of riboflavin nutriture in man has generally been estimated by using either fluorometric (1-4) or microbiological assay methods to measure the amount of rib oflavin present in urirfe, plasma or serum, erythrocytes, or whole blood, while these measurements correlate with the clinical signs associated with ribOflavin deficiency.

Analysis of Riboflavin and its Derivatives in Biologic Fluids and Tissues

In most analytical procedures, it is necessary to treat natural products with acid or enzymes for maximal values, that is, to liberate riboflavin from its protein moiety thus making it more readily extractable for assay.

Experimental Dietary and Antagonist-Induced Human Riboflavin Deficiency

By the mid-1930s many clinical symptoms and signs attributable to riboflavin deficiency were described in pellagrins and in other dietary deficiencies. (7–9,31,46,58,62,73,82) The specificity of

Factors affecting the absorption of riboflavin in man.

The urinary recovery of rib oflavin as a function of dose after oral administration to fasted normal humans shows that the process responsible for the absorption of this vitamin is saturable, and the site specificity and saturability of riboflavin absorption suggest that the vitamin is absorbed by specialized transport rather than by passive diffusion.

Plasma protein binding of riboflavin and riboflavin-5'-phosphate in man.

There is indication that the interaction between FR and albumin is nonionic, but that electrostatic forces contribute appreciably to the binding of FMN to albumin.

Synthesis of riboflavin nucleotides by mature human erythrocytes.

The rate of incorporation of radioactive riboflavin into red blood cells is the same whether the cells are suspended in plasma or in a phosphate-saline-glucose medium, suggesting that the FAD concentration in the red cells increased during the incubation.

Riboflavin activity in normal and leukemic human leukocytes and nuclei.

Riboflavin has been determined microbiologically in human leukocytes isolated in high purity by the phytohemagglutinin technique and it is possible in most instances to distinguish between the white cells of the several forms of acute leukemia and thus make a differential diagnosis.


hp://w w w .jb.org/ D ow nladed from Lowry

  • Biochem. J.,
  • 1945