Fluorometric measurements of riboflavin and its natural derivatives in small quantities of blood serum and cells.

@article{Burch1948FluorometricMO,
  title={Fluorometric measurements of riboflavin and its natural derivatives in small quantities of blood serum and cells.},
  author={Helen B. Burch and Otto A. Bessey and Oliver H. Lowry},
  journal={The Journal of biological chemistry},
  year={1948},
  volume={175 1},
  pages={
          457-70
        }
}

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References

hp://w w w .jb.org/ D ow nladed from Lowry

  • Biochem. J.,
  • 1945