Fluorometric measurement of 5-aminolevulinic acid in serum.

  title={Fluorometric measurement of 5-aminolevulinic acid in serum.},
  author={Chul Lee and Xian Qiao and Douglas E. Goeger and Karl E. Anderson},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  volume={347 1-2},
BACKGROUND Measurement of 5-aminolevulinic acid (ALA) in serum is potentially useful in acute porphyrias, lead poisoning and hereditary tyrosinemia. Because levels of ALA in serum are about 100 times less than in urine, a highly sensitive method is required. We describe a simple and sensitive fluorometric method that does not require HPLC. METHODS ALA is separated from serum using a cation-exchange resin (DOWEX 50WX8-400), followed by addition of acetylacetone and formaldehyde to produce a… CONTINUE READING


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