Fluorometric detection of ca(2+) based on an induced change in the conformation of sol-gel entrapped parvalbumin.

@article{Flora1998FluorometricDO,
  title={Fluorometric detection of ca(2+) based on an induced change in the conformation of sol-gel entrapped parvalbumin.},
  author={Karl P. Flora and John D Brennan},
  journal={Analytical chemistry},
  year={1998},
  volume={70 21},
  pages={4505-13}
}
We report the development of a fluorometric detection strategy for Ca(2+) based on induced changes in the conformation of cod III parvalbumin entrapped within a sol-gel processed glass. The detection scheme utilizes a fluorescent allosteric signal transduction (FAST) strategy wherein conformational changes induced by Ca(2+) binding result in alterations in the intrinsic fluorescence from the single tryptophan residue at position 102. Intrinsic fluorescence was also used to examine chemically… CONTINUE READING