Fluorometric assessments of mitochondrial function and viability in cryopreserved bovine spermatozoa.

@article{Garner1997FluorometricAO,
  title={Fluorometric assessments of mitochondrial function and viability in cryopreserved bovine spermatozoa.},
  author={Duane L. Garner and Cheryl A. Thomas and Hannes Joerg and James M Dejarnette and Clifton Eugene Marshall},
  journal={Biology of reproduction},
  year={1997},
  volume={57 6},
  pages={1401-6}
}
Mitochondrial function and sperm viability were quantified in samples of cryopreserved bovine spermatozoa from 12 bulls using fluorometric techniques. The active mitochondria of the spermatozoa were fluorescently stained using three different fluorophores: rhodamine 123 (R123), 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyan ine iodide (JC-1) or MitoTracker Green FM (MITO). The stained spermatozoa, and companion aliquots that had been stained with SYBR-14 (a living-cell… CONTINUE READING
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